Using Thin Layer Chromatography As A Drug Screen Technique

Drug screening, which is generally used in criminal situations, health care and the workplace has recently become more common. The ease of use and fast results have increased the use of immunoassays; however, these can cause false positive results. This can lead to severe consequences if they are not confirmed by secondary analysis, such as gas chromatography-mass spectrometry (GCMS). The drugs screened during this experiment were ketamine, methadone, diazepam, codeine and imipramine. This report discusses the use of thin layer chromatography as a drug screen technique. The results obtained from this are compared to the secondary analysis from GCMS which show that the drugs from the unknown sample were ketamine, methadone and codeine. The main techniques are examined to gain a better understanding of the methods used. Potential false positive and false negative results that can occur are also discussed. Introduction A drug screen is an analysis of a physiological fluids such as urine, blood, sweat, or saliva to determine the presence or absence of specified drugs or their metabolites. The main applications of drug testing include police officers testing for the presence and concentration of alcohol in the blood, detection of the presence of performance enhancing drugs in sport and employers screening staff for illegal drugs. When there are a large number of samples for drug screening, immunoassay techniques are favoured as they are cheaper and faster. This isShow MoreRelatedDetection or Identification of Trace Amounts of Explosives1624 Words   |  6 PagesThe detection and/or identification of trace amounts of explosives. The review of each method should include a description of the way the method works and the advantages and drawbacks of the technique. Introduction An explosive, are reactive substances that contain a large amount of potential energy that can yield an explosion if they are released rapidly, they are accompanied by the production of light, heat, sound, and pressure. Mainly used in the mining industry for extraction of mineralsRead MoreAmmonium Sulfate From Waste Water3280 Words   |  14 PagesCamelford water pollution incident Unit Coordinator: Anne Ng Student: Saud Boresli 3908933 Table of Contents 1.0 What happened at Camelford? 3 2.0 Expulsion of Ammonium sulfate from waste water 4 2.1.1 Membrane Technology 5 2.1.2. Layer Bioreactor 5 2.1.3. Particle Exchange 6 2.1.4. Reverse Osmosis 7 2.2. Recuperation of ammonium sulfate from the concentrates 8 2.2.1. Vanishing Ponds 8 2.2.2. Precipitation strategy 8 2.2.3. Crystallization strategy 9 2.3 Winterization process 10 3Read MoreQuality Improvement328284 Words   |  1314 Pagesof Manufacturing Engineering, was Professor of Mechanical Engineering, and was Director of the Program in Industrial Engineering. Dr. Montgomery has research and teaching interests in engineering statistics including statistical quality-control techniques, design of experiments, regression analysis and empirical model building, and the application of operations research methodology to problems in manufacturing systems. He has authored and coauthored more than 190 technical papers in these fields